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目的探讨白藜芦醇对Aβ25-35诱导PC12细胞损伤的保护作用。方法用不同浓度的Aβ25-35处理PC12细胞,建立细胞毁损模型,然后加入不同浓度的白藜芦醇,在显微镜下观察PC12细胞形态的变化及突起生长情况。采用MTT检测技术观察PC12细胞的生长活性,采用酶标仪检测乳酸脱氢酶(LDH)的活性,采用瑞士-吉姆萨染色和流式细胞术检测细胞凋亡情况。结果损伤组加入Aβ25-35,24 h后细胞形态不规整,突起出现不同程度的肿胀破裂,48 h后见不到完整的突起结构;保护组加入白藜芦醇预孵育后明显延缓突起损伤,24 h后仍能观察到比较完整的突起结构,48 h后远端发生轻微断裂。保护组细胞突起长度和D(λ)值明显大于损伤组(P<0.01),细胞凋亡数以及LDH活性明显低于损伤组(P<0.01)。结论白藜芦醇对Aβ25-35诱导的PC12细胞损伤具有明显的保护作用。
Objective To investigate the protective effect of resveratrol on Aβ25-35-induced PC12 cell injury. Methods PC12 cells were treated with different concentrations of Aβ25-35 to establish a model of cell damage. Resveratrol was then added in different concentrations to observe the morphological changes of PC12 cells and the growth of the cells. The growth of PC12 cells was observed by MTT assay. The activity of lactate dehydrogenase (LDH) was detected by microplate reader. The apoptosis of PC12 cells was detected by Swiss Giemsa staining and flow cytometry. Results Aβ25-35 was added into the injured group for 24 h, and the cell morphology was irregular. The protuberant protuberances showed different degrees of swelling and rupture, and the intact protuberances were not seen after 48 h. The pretreatment with resveratrol in the protective group significantly delayed the neurite outgrowth, After 24 h, more intact protuberances were still observed, and a slight distal rupture occurred 48 h later. The length of protuberance and the value of D (λ) in protective group were significantly higher than those in injured group (P <0.01). The number of apoptotic cells and LDH activity in protective group were significantly lower than those in injured group (P <0.01). Conclusion Resveratrol has significant protective effect on Aβ25-35-induced PC12 cell injury.