用新近发展的荧光示踪方法和照明方法,用光学显微镜对单个小分子进行了目视和光电方法的观察。 8～100个荧光素异硫氰酸盐分子结合成一个分子量为20000的聚乙烯亚胺,然后结合成一个γ球朊。然后用强度高到足以在观察期间产生完全光化漂白的光对试剂照明并加以探测。这样,产生短的荧光脉冲,它含有最大的可能的荧光信号,能产生约5～10×10~5光子/试剂分子的荧光素标记。 Using a newly developed fluorescent tracer method and illumination method, a single small molecule was observed with a light microscope using visual and photoelectric methods. Eight to 100 fluorescein isothiocyanate molecules combine to form a polyethyleneimine with a molecular weight of 20,000 and then combine into a gamma globulin. The reagent is then illuminated and probed with light of sufficient intensity to produce fully photobleached bleach during the observation. In this way, a short fluorescence pulse is generated which contains the largest possible fluorescence signal and produces a fluorescein label of about 5 to 10 x 10 5 photons / reagent molecule.