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目的:探讨间隙连接蛋白43(Cx43)和E-钙粘素(E-cad)在口腔鳞癌中的表达及其相关性;细胞分化诱导剂全反式维甲酸(ATRA)对人舌鳞癌细胞株(Tca8113)中Cx43和E-cad表达的影响。方法:应用免疫组织、细胞化学结合免疫印迹(western blot)技术,观察口腔鳞癌组织中及ATRA对Tca8113细胞株进行培养及分化诱导后Cx43和E-cad的变化。结果:正常口腔鳞状上皮中Cx43和E-cad主要表达于细胞膜;在鳞癌组织中,Cx43和E-cad细胞膜表达与组织的分化程度、转移之间有显著性差异(Cx43:х2=7.42、12.43,p<0.05、p<0.01;E-cad:х2=7.79、9.688,p<0.05、p<0.01)。在鳞癌组织同一标本中Cx43和E-cad表达具有正相关性和一致性(r=0.577,p<0.0005);舌鳞癌细胞经ATRA诱导后,Cx43和E-cad蛋白表达增加。结论:Cx43和E-cad在口腔鳞癌的发生和发展过程中具有协同作用;ATRA可以通过上调Cx43和E-cad的表达,实现对肿瘤生长的抑制。
AIM: To investigate the expression of connexin 43 (Cx43) and E-cadherin (E-cad) in oral squamous cell carcinoma and their correlation. The effect of ATRA on human tongue squamous cell carcinoma Effect of Cx43 and E-cad on cell lines (Tca8113). Methods: The expressions of Cx43 and E-cad in Tca8113 cells after oral administration of Tca8113 cells were detected by immunohistochemistry and western blot. Results: Cx43 and E-cad in normal oral squamous epithelium were mainly expressed in the cell membrane. There was significant difference between the expression of Cx43 and E-cad in the squamous cell carcinoma and the histological differentiation and metastasis (Cx43: х2 = 7.42 , 12.43, p <0.05, p <0.01; E-cad: х2 = 7.79,9.688, p <0.05, p <0.01). The expression of Cx43 and E-cad was positively correlated and consistent in the same specimen of squamous cell carcinoma (r = 0.577, p <0.0005). The expression of Cx43 and E-cad in tongue squamous carcinoma cells was increased after ATRA induction. CONCLUSION: Cx43 and E-cad have synergistic effects in the development and progression of oral squamous cell carcinoma. ATRA can inhibit the tumor growth by up-regulating the expression of Cx43 and E-cad.