肾小球基底膜PAMS染色方法的应用体会

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病理技术中,显示肾小球基底膜多采用过碘酸六铵银(PAMS)染色法,该法在肾小球疾病的病理诊断中具有重要的参考价值。我们在实际工作中发现,传统的PAMS染色法染色效果不佳,基底膜染色较浅、背景较深,染色时间过长。经过大量实验,我们对染液配方、染色时间及温度等进行了 Pathology, glomerular basement membrane showed more use of hexamine silver (PAMS) staining method, the method in the pathological diagnosis of glomerular disease has an important reference value. We found in the actual work, the traditional staining method PAMS dyeing poor results, the basement membrane staining lighter, darker background, dyeing time is too long. After a large number of experiments, we dye solution, dyeing time and temperature were carried out
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