目的 :观察安美汀、红霉素在体外对铜绿假单胞菌菌膜的作用。方法 ;将聚乙烯膜和绿脓杆菌置LB培养基中培养 4 8h ,扫描电镜观察有菌膜形成时取出聚乙烯膜 ,漂洗后分别放入含有不同浓度安美汀和红霉素的LB培养基中 ,2 4h后观察菌膜情况 ,并将培养液接种至LB琼脂平板观察是否有菌落形成。结果 :聚乙烯膜和铜绿假单胞菌共培养 4 8h ,扫描电镜观察到有菌膜形成 ;浓度为 10 0、30 0和 5 0 0 μg/ml的安美汀和浓度为 5 0、10 0和 30 0 μg/ml的红霉素作用 2 4h,聚乙烯膜上的铜绿假单胞菌菌膜均消失 ,培养液接种至LB琼脂平板无菌落形成。结论 :聚乙烯膜和铜绿假单胞菌共培养 4 8h可成功制作铜绿假单胞菌菌膜的体外模型 ;较高浓度的安美汀和红霉素对伴有菌膜形成的铜绿假单胞菌有杀灭作用 OBJECTIVE: To observe the effect of amitin and erythromycin on P. aeruginosa membrane in vitro. Methods: Polyethylene membrane and Pseudomonas aeruginosa were cultured in LB medium for 48 h. The membrane was taken out by scanning electron microscopy and the polyethylene membrane was removed. After rinsing, LB medium containing different concentration of amphotericin and erythromycin In 24 hours after the observation of bacterial conditions, and the culture medium was inoculated to LB agar plate to observe whether there is colony formation. Results: Polyethylene membrane and Pseudomonas aeruginosa were co-cultured for 48 h, and the formation of bacterial membrane was observed by scanning electron microscopy. At the concentrations of 10 0,30 0 and 5 0 0 μg / ml, And 30 0 μg / ml of erythromycin for 24 hours, the polyethylene membrane of Pseudomonas aeruginosa bacteria disappeared, the culture medium was inoculated into LB agar plate formed colony. Conclusion: Polyethylene membrane and Pseudomonas aeruginosa co-culture 48 h can be successfully produced in vitro models of Pseudomonas aeruginosa membrane; higher concentrations of the combination of ampicillin and erythromycin with the formation of bacterial membrane formation of Pseudomonas aeruginosa Bacteria have a killing effect