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反向遗传学方法是研究RNA病毒的主要方法,痘苗病毒载体则是感染性克隆技术的主要媒介,目前这一技术已趋向成熟并扩大其应用领域及对象。本文对痘苗病毒载体的构建、在冠状病毒反向遗传学中的应用情况以及目前该领域的进展作一综述。
The reverse genetics method is the main method to study RNA viruses. Vaccinia virus vector is the main vector of infectious clone technology. At present, this technology has matured and expanded its application field and object. This article reviews the construction of vaccinia virus vectors, the application in coronavirus reverse genetics and the current progress in this field.