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目的通过联合检测PTENmRNA,P73mRNA在肝外胆管癌中的表达,探讨其与肿瘤生物学特性的关联及临床意义。方法应用石蜡包块切片组织原位杂交染色法检测42例肝外胆管癌中PTENmRNA,P73mRNA的表达,并以同期20例慢性胆管炎作对照。结果PTENmRNA阳性表达率为57.1%(24/42),显著低于良性对照组(为100%);P73mRNA表达率为54.8%(23/42),显著高于良性对照组(为0)。经统计学分析PTENmRNA,P73mRNA的表达都分别与肝外胆管癌的临床病理分期及预后显著相关(P<0.05);PTENmRNA的表达还与肝外胆管癌的组织学分级显著相关(P<0.05)。尚不能认为PTENmRNA,P73mRNA在肝外胆管癌的表达两者有相关关系。结论PTEN,P73基因在肝外胆管癌的发生发展过程中可能起重要作用,联合检测PTENmRNA,P73mRNA在肝外胆管癌中的表达,有助于了解其生物学特性,为预后判断提供参考指标。
Objective To detect the expression of PTEN mRNA and P73 mRNA in extrahepatic cholangiocarcinoma and to investigate the relationship between PTEN mRNA and tumor biological characteristics and its clinical significance. Methods The expression of PTEN mRNA and P73 mRNA in 42 cases of extrahepatic cholangiocarcinoma were detected by paraffin-embedded tissue in situ hybridization staining. Twenty cases of chronic cholangitis were used as controls. Results The positive expression rate of PTEN mRNA was 57.1% (24/42), which was significantly lower than that of benign control group (100%). The expression rate of P73 mRNA was 54.8% (23/42), which was significantly higher than that of benign control group (0). Statistical analysis of PTEN mRNA and P73 mRNA expression were significantly associated with the clinical pathological stage and prognosis of extrahepatic cholangiocarcinoma, respectively (P<0.05); PTEN mRNA expression was also significantly associated with the histological grade of extrahepatic cholangiocarcinoma (P<0.05). . It cannot be considered that there is a correlation between the expression of PTEN mRNA and P73 mRNA in extrahepatic cholangiocarcinoma. Conclusion The PTEN and P73 genes may play an important role in the development of extrahepatic cholangiocarcinoma. The combined detection of PTEN mRNA and P73 mRNA in extrahepatic cholangiocarcinoma may help to understand its biological characteristics and provide a reference for prognosis.