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为验证壳聚糖的自发荧光机理,以顺-2-甲基-2-丁烯醛和2-氨基-2-甲基丙烷为原料,在-14℃无水无氧条件下合成了具有-C==N-C==C-结构单元的产物,通过傅立叶红外光谱和液质联用技术进行结构确认,并对合成产物进行了荧光光谱扫描和激光共聚焦光谱扫描分析.结果表明,合成的物质在488nm激发下,于510～540和570～600nm波段采集到了稳定的荧光,与壳聚糖荧光微球的荧光波长一致.据此可推断壳聚糖微球的自发荧光主要源于-C==N-C==C-共轭结构单元.
In order to verify the mechanism of autofluorescence of chitosan, cis-2-methyl-2-butenal and 2-amino-2-methylpropane were synthesized under the condition of -14 ℃ anhydrous and anaerobic conditions. The structure of C == NC == C-unit was confirmed by Fourier transform infrared spectroscopy and liquid chromatography-mass spectrometry (LC-MS / MS), and the synthesized products were analyzed by fluorescence spectrum and confocal laser scanning microscopy.The results showed that the synthesized material Under the excitation of 488nm, stable fluorescence was collected in 510-540 and 570-600nm bands, which was consistent with the fluorescence wavelength of chitosan fluorescent microspheres.Accordingly, the autofluorescence of chitosan microspheres mainly derived from -C = = NC == C-conjugated structural unit.