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目的 探讨深低温冻存角膜复温后内皮细胞活性变化的规律。方法 冻存牛角膜复温后 ,分别行器官培养(A组 )及optisol保存 (B组 ) ,检测内皮细胞密度、存活率 (ESR)及形态。结果 A组培养 6h密度下降了 71 2 % ,2 4hESR明显下降 (2 7 2 % ) ;B组保存 72h密度下降了 73 6% ,ESR明显下降 (3 7 2 % )。结论 深低温冻存可造成内皮细胞的潜伏性损伤 ,判定其活性应在复温后组织培养 6h后进行。复温后Optisol保存能改善细胞的潜在性损伤
Objective To investigate the changes of endothelial cell activity after corneal cryopreservation. Methods After cryopreservation of cornea, the organ culture (group A) and optisol (group B) were used respectively to detect the density, survival rate and morphology of endothelial cells. Results The density of group A decreased 71 2% and 24 h decreased significantly (72.2%) in group A at 6 hours. In group B, the density decreased 73 6% at 72 h and the ESR decreased significantly (37 2%). Conclusion Cryogenic cryogenic storage can cause latent damage of endothelial cells, and its activity should be determined after tissue culture 6h after rewarming. Optisol preservation after rewarming can improve the potential damage of cells