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目的研究α-Synuclein(α-syn)及磷酸化α-syn(P-syn)在功能性便秘患者结肠黏膜的表达。方法应用免疫荧光双重标记和显微图像分析技术,比较21例功能性便秘和22例对照组结肠黏膜α-syn和P-syn的表达情况。结果 2组肠黏膜神经元及胶质细胞均有α-syn和P-syn表达,存在共定位。功能性便秘组结肠黏膜层及黏膜下层α-syn积分光密度(IOD)分别为1 513.50±93.21和1 398.65±74.32,显著高于对照组(1 027.10±57.81和995.03±68.32),差异有统计学意义(P<0.05)。功能性便秘组结肠黏膜层及黏膜下层P-syn的IOD(790.25±39.41和432.09±18.99)显著高于对照组(535.87±35.72和310.90±27.26),差异有统计学意义(P<0.05)。结论结肠黏膜神经元及胶质细胞α-syn的聚集及磷酸化可能与功能性便秘发病机制相关。
Objective To study the expression of α-syn (α-syn) and phosphorylated α-syn (P-syn) in colonic mucosa of patients with functional constipation. Methods The expression of α-syn and P-syn in colonic mucosa of 21 cases of functional constipation and 22 cases of control group were compared by immunofluorescence double labeling and microscopic image analysis. Results The expressions of α-syn and P-syn in both intestinal mucosa neurons and glial cells were co-localized. The integral optical density (IOD) of α-syn in colonic mucosa and submucosa of functional constipation group were 1 513.50 ± 93.21 and 1 398.65 ± 74.32, respectively, which were significantly higher than that of the control group (1027.10 ± 57.81 and 995.03 ± 68.32) Significance (P <0.05). The IOD (790.25 ± 39.41 and 432.09 ± 18.99) of functional constipation group in colonic mucosa and submucosal P-syn were significantly higher than those in control group (535.87 ± 35.72 and 310.90 ± 27.26), respectively. The difference was statistically significant (P <0.05). Conclusion The aggregation and phosphorylation of α-syn in colonic mucosa neurons and glial cells may be related to the pathogenesis of functional constipation.