论文部分内容阅读
分别以5、10、15、20、25Gy电子束剂量辐照百合品种Starfighter和Tiber的鳞茎,发现该剂量梯度明显抑制百合当代的营养生长和生殖生长,但不影响新鳞茎定植后第2代正常营养生长和开花,而且在C-15Gy第2代中发现花朵颜色变深、花瓣斑点长突起变短的变异株。以百合幼嫩叶片作为材料,应用RAPD技术分析不同剂量电子束辐照的扩增结果。11个试验组中,有5个随机引物扩增出清晰谱带282条,其中,32条为多态性谱带,多态率为11.35%。RAPD结果显示,电子束辐照百合鳞茎能够引起百合DNA变异,并且辐照剂量与诱变频率呈正相关关系,此外诱变频率还与试验品种、辐照部位及所处的生长期有关。
The bulbs of Starfighter and Tiber were irradiated with 5, 10, 15, 20 and 25Gy electron beam doses, respectively. The dose gradient obviously inhibited the contemporary vegetative and reproductive growth of lily, but did not affect the second generation of normal bulbs Vegetative growth and flowering, and in the second generation of C-15Gy found that the flower color darker, petal blob mutant shorter mutant. Using Lily young leaves as material, RAPD technique was used to analyze the amplification results of different doses of electron beam irradiation. Of the 11 experimental groups, there were 282 clear bands amplified by 5 random primers, of which 32 were polymorphic and the polymorphism rate was 11.35%. RAPD results showed that electron beam irradiation lily bulbs can cause lily DNA variation, and radiation dose and mutation frequency was positively correlated, in addition mutagenic frequency also with the test species, the irradiation site and the growth period.