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目的：在大肠杆菌中高效表达抗乙肝表面抗原的单链抗体。方法：通过ＰＣＲ将单链抗体基因重组到原核细胞表达载体ｐＴ７中，构建单链抗体高效表达载体ｐＴ７ＳＣ。将ｐＴ７ＳＣ质粒转化大肠杆菌ＢＬ２１（ＤＥ３），ＩＰＴＧ诱导进行表达。结果：获得了ＳｃＦｖ的高效表达，表达产物占菌体总蛋白的２８％以上。竞争抑制性ＥＬＩＳＡ检测诱导后的细菌培养上清，证明所表达的ＳｃＦｖ具有抗体活性。进一步将ｃＭｙｃ十肽连接在ＳｃＦｖ的羧基端，使所表达的ＳｃＦｖ易于检测。结论：在大肠杆菌高效表达抗乙肝ＳｃＦｖ，将促进小分子抗体的应用
OBJECTIVE: To express single chain antibodies against hepatitis B surface antigen efficiently in E. coli. Methods: Single chain antibody gene was recombined into prokaryotic expression vector pT7 by PCR to construct pT7SC. The pT7SC plasmid was transformed into E. coli BL21 (DE3) and induced by IPTG. Results: The high expression of ScFv was obtained. The expressed product accounted for more than 28% of total bacterial protein. Competitive inhibition ELISA was used to detect the induced bacterial culture supernatants, demonstrating that the expressed ScFv has antibody activity. The c-Myc ten peptide was further linked to the carboxyl terminus of ScFv to make the expression of ScFv easy to detect. Conclusion: High expression of anti-hepatitis B virus ScFv in Escherichia coli will promote the application of small molecule antibodies