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以溶菌酶作为模型蛋白,主要利用光谱法研究了埃洛石纳米管与溶菌酶之间的相互作用。荧光光谱结果表明向溶菌酶体系中加入埃洛石纳米管会出现荧光猝灭现象,猝灭机理符合静态猝灭规律。共振光散射强度的增加可能与埃洛石纳米管-溶菌酶复合物的形成而导致的分子尺寸的增加有关,这与紫外-可见吸收光谱的变化和静态猝灭机理相一致。同步荧光光谱分析表明两者之间的相互作用可能发生在色氨酸所处位置附近,作用过程使溶菌酶的二级结构发生变化,分子链错误折叠,加入浓度为100mg/L和200mg/L的埃洛石纳米管时,通过圆二色谱数据计算出分别导致α-螺旋的含量降低3.28%和6.89%。
Using lysozyme as a model protein, the interaction between halloysite and lysozyme was mainly studied by spectroscopy. Fluorescence spectroscopy results showed that adding halloysite nanotubes to the lysozyme system showed fluorescence quenching, and the quenching mechanism accorded with the static quenching law. The increase of resonance light scattering intensity may be related to the increase of molecular size caused by the formation of halloysite nanotube-lysozyme complex, which is consistent with the change of UV-visible absorption spectrum and the static quenching mechanism. Synchronous fluorescence spectroscopy analysis showed that the interaction between the two may occur in the vicinity of tryptophan, the secondary structure of lysozyme changes, the molecular chain misfolded, adding the concentration of 100mg / L and 200mg / L Of halloysite nanotubes, the content of a-helix decreased by 3.28% and 6.89%, respectively, as calculated by circular dichroism data.