论文部分内容阅读
目的研究白细胞介素-6(IL-6)、髓样细胞白血病-1(MCL-1)蛋白及MCL-1mRNA在宫颈癌、癌前病变及正常宫颈组织中的表达情况及其相关性。方法采用免疫组化法检测30例宫颈鳞癌、10例宫颈上皮内瘤变(CIN)及10例正常宫颈组织中IL-6和MCL-1蛋白表达情况,采用逆转录PCR法检测组织中MCL-1 mRNA的表达情况。结果IL-6和MCL-1蛋白在宫颈癌组织中的表达高于正常宫颈组织及CIN组织(P<0.01),且IL-6和MCL-1蛋白的表达呈正相关性(r=0.566,P<0.01);MCL-1蛋白与MCL-1mRNA表达呈正相关性(r=0.772,P<0.01)。结论IL-6和MCL-1的表达在宫颈癌的发生、发展过程中起着共同调节作用,这一发现有助于对早期癌前病变的筛查,并为宫颈癌的治疗提供了一个细胞因子靶点。
Objective To investigate the expression of interleukin-6 (IL-6), myeloid leukemia-1 (MCL-1) protein and MCL-1 mRNA in cervical cancer, precancerous lesions and normal cervical tissues. Methods The expressions of IL-6 and MCL-1 in 30 cases of cervical squamous cell carcinoma, 10 cases of cervical intraepithelial neoplasia (CIN) and 10 cases of normal cervical tissue were detected by immunohistochemistry. The expression of MCL -1 mRNA expression. Results The expression of IL-6 and MCL-1 protein in cervical cancer tissues was higher than that in normal cervical tissues and CIN tissues (P <0.01), and the expression of IL-6 and MCL-1 protein was positively correlated (r = 0.566, P <0.01). There was a positive correlation between MCL-1 protein and MCL-1 mRNA expression (r = 0.772, P <0.01). Conclusion The expressions of IL-6 and MCL-1 play a common regulatory role in the development and progression of cervical cancer. This finding is helpful for the screening of precancerous lesions and provides a cell for the treatment of cervical cancer Factor target.