论文部分内容阅读
目的研究补体成分C9、肿瘤坏死因子-α(TNF-α)在大鼠实验性脑出血后血肿周围的表达情况,探讨C9、TNF-α在脑出血后脑水肿中的作用及应用眼镜蛇毒因子(CVF)干预后的影响。方法采用立体定向技术,将自体不凝血注入大鼠尾状核制备脑出血模型,将动物分为假手术组、出血组和CVF干预组,分别在不同时间断头取脑,连续切片作C9、TNF-α免疫组化染色和HE染色,并进行脑组织含水量测定(干湿重法)。结果脑出血后2h开始表达C9,24 h达高峰;TNF-α24 h表达明显增加,48 h达高峰,各组与假手术组之间比较有差异(P<0.05);TNF-α的表达与C9的表达呈正相关关系(r=0.833,P<0.05);血肿周围脑组织含水量在ICH后2 h开始增加,24~72 h达高峰(P<0.01),此后逐渐回落,1周基本恢复正常水平,对侧半球相应部位及假手术对照组脑组织含水量没有明显变化;经CVF干预后,血肿周围组织C9、TNF-α表达量明显下降,干预组与出血组之间比较有显著差异(P<0.01)。CVF干预组脑组织含水量明显低于常规ICH组(P<0.01)。结论脑出血后补体级联激活C9及TNF-α表达明显增加,CVF干预后,C9及TNF-α表达下降,脑水肿减轻,能达到神经保护作用。
Objective To investigate the expression of complement C9 and tumor necrosis factor-α (TNF-α) in the perihematoma after experimental intracerebral hemorrhage in rats and to explore the role of C9 and TNF-α in cerebral edema after intracerebral hemorrhage. CVF) after the intervention. Methods Stereotactic technique was used to inject intracerebral hemorrhage into rat caudate nucleus to prepare intracerebral hemorrhage model. Animals were divided into sham operation group, hemorrhage group and CVF intervention group. The animals were sacrificed at different time, TNF-α immunohistochemical staining and HE staining, and determination of brain water content (wet and dry weight method). Results The levels of TNF-α at 24 h and 2 h after intracerebral hemorrhage reached the peak at C9 and 24 h, and reached the peak at 48 h and reached the peak at 48 h (P <0.05). The expressions of TNF- (R = 0.833, P <0.05). The water content of brain tissue around hematoma began to increase at 2 h after ICH and peaked at 24 to 72 h (P <0.01), and then gradually dropped back to a level of 1 week At normal level, there was no significant change in brain water content of the corresponding hemisphere and sham-operated control group. After CVF intervention, the expression of C9 and TNF-α in perihematoma tissue decreased significantly, and there was significant difference between intervention group and hemorrhage group (P <0.01). CVF intervention group brain water content was significantly lower than the conventional ICH group (P <0.01). Conclusions The expression of C9 and TNF-α induced by complement cascade increases obviously after intracerebral hemorrhage. After CVF intervention, the expression of C9 and TNF-α is decreased and the brain edema is relieved.