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迄今,对乙型病毒性肝炎慢性化和重性化的机制仍缺乏了解,近年发现免疫细胞经刺激活化后发生凋亡的现象(AICD)可能是形成免疫耐受的一种机制.我们利用正常人与慢性乙型病毒性肝炎患者外周血 PBMC 在 PHA 刺激下短期培养,通过流式细胞仪检测凋亡情况并相互比较,从而探讨 AICD 在乙型病毒性肝炎慢性化和重型化中的作用.1 材料和方法1.1 材料住院慢性乙型肝炎患者20例均符合1995年第二次全国病毒性肝炎会议诊断标准.男18例,女2例,年龄岁22～44岁,平均34岁±5岁.其中慢性肝炎14例,慢性重型肝炎6例.10例健康人取自我院输血科献血员,男8例,女2例,年龄28岁～45岁,平均36岁±6岁.无菌抽取肝素抗凝全血
To date, there is still a lack of understanding of the mechanism of chronic hepatitis B virus infection. In recent years, it has been found that apoptosis of immune cells triggered by stimulation (AICD) may be a mechanism for the formation of immune tolerance. Peripheral blood PBMC of human and chronic hepatitis B patients were cultured in PHA for a short time, apoptosis was detected by flow cytometry and compared with each other to explore the role of AICD in chronic and heavy-handedness of hepatitis B virus. 1 Materials and Methods 1.1 Materials 20 cases of hospitalized patients with chronic hepatitis B are in line with the 1995 second national diagnostic criteria of viral hepatitis meeting, 18 males and 2 females, aged 22 to 44 years old, with an average of 34 years old ± 5 years , Of which 14 cases of chronic hepatitis and 6 cases of chronic severe hepatitis.The blood of 10 blood donors was collected from blood transfusion department of our hospital, including 8 males and 2 females, aged from 28 to 45 years (average 36 years ± 6 years) Extraction of heparin anticoagulant whole blood