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目的探讨5种清热解毒中药单体对SMMC-7721肝癌细胞增殖和对H22荷瘤小鼠移植瘤的抑制作用。方法SMMC-7721人肝癌细胞分别经不同浓度的迷迭香酸、黄芩苷、橙黄决明素、绿原酸和黄连素作用48 h后,四甲基偶氮唑盐法检测细胞增殖抑制率并筛选出有效中药单体。采用H22肝癌细胞建立小鼠移植性肝癌模型,给予H22荷瘤小鼠不同浓度的迷迭香酸(300 mg/kg、150 mg/kg、75 mg/kg)和黄芩苷(200 mg/kg、100 mg/kg、50 mg/kg)10 d后,剥取瘤块、胸腺、脾脏称重,并计算抑瘤率、胸腺指数和脾脏指数。结果迷迭香酸500 mg/L、250 mg/L、125 mg/L浓度组和黄芩苷500 mg/L、250 mg/L浓度组吸光度A值均明显低于对照组(P<0.05),且细胞增殖抑制率均达到30%以上;橙黄决明素及黄连素组的细胞增殖抑制率均低于30%,绿原酸组细胞增殖抑制率<0。各剂量迷迭香酸组的瘤重均较模型组明显下降(P<0.05),对H22移植瘤的抑制率分别为48.2%、43.0%和39.0%,迷迭香酸各剂量组的胸腺指数和脾脏指数与模型组比较,差异无统计学意义(P>0.05)。300 mg/kg黄芩苷组肿瘤重均低于模型组(P<0.05),抑瘤率达到55.7%,黄芩苷各剂量组的脾脏、胸腺指数与模型组比较无显著性差异(P>0.05)。结论迷迭香酸和黄芩苷体外对SMMC-7721肝癌细胞和体内对H22移植瘤均有明显的抑制作用。
Objective To investigate the inhibitory effects of five kinds of Qingrejiedu traditional Chinese medicine monomer on the proliferation of SMMC-7721 hepatoma cells and the transplanted tumor of H22 tumor-bearing mice. Methods SMMC-7721 human hepatocellular carcinoma cells were treated with different concentrations of rosmarinic acid, baicalin, orange cassia seed, chlorogenic acid and berberine for 48 h, respectively, and the cell proliferation inhibition rate Select effective Chinese medicine monomer. H22 hepatoma cells were used to establish mouse model of transplanted liver cancer. Rosiglitazone (300 mg / kg, 150 mg / kg, 75 mg / kg) and baicalin (200 mg / kg, 100 mg / kg, 50 mg / kg) for 10 days. The tumor mass, thymus and spleen were removed and weighed. The tumor inhibition rate, thymus index and spleen index were calculated. Results The absorbance A value of rosmarinic acid at concentrations of 500 mg / L, 250 mg / L, 125 mg / L and baicalin 500 mg / L and 250 mg / L were significantly lower than those of the control group The inhibitory rate of cell proliferation reached more than 30%. The inhibitory rates of cell proliferation were lower than 30% in the groups of Huanghuang Jindingin and berberine, and the inhibition rates of cell proliferation in the group of chlorogenic acid were less than 0%. The tumor weight of rosmarinic acid group at each dose was significantly lower than that of the model group (P <0.05), and the inhibitory rates of H22 transplanted tumor were 48.2%, 43.0% and 39.0% respectively. The thymus index And spleen index compared with the model group, the difference was not statistically significant (P> 0.05). The tumor weight in 300 mg / kg baicalin group was lower than that in model group (P <0.05), and the tumor inhibition rate was 55.7%. The spleen and thymus index in baicalin groups showed no significant difference compared with model group (P> 0.05) . Conclusion Rosmarinic acid and baicalin can inhibit SMMC-7721 hepatoma cells and H22 xenografts in vivo in vitro.