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目的 :探讨新型化合物木脂素1对人肝癌Hep G2细胞增殖及凋亡的影响。方法 :不同质量浓度的木脂素1处理Hep G2细胞后,应用MTT法检测各组Hep G2细胞的增殖情况,FCM法检测各组Hep G2细胞的细胞周期分布及凋亡情况,蛋白质印迹法检测各组Hep G2细胞中凋亡相关蛋白cleaved caspase 3蛋白的表达水平。结果 :1.25~20μg/m L木脂素1处理Hep G2细胞24、48和72 h后,细胞增殖被显著抑制,并呈时间-剂量依赖性(P值均<0.05)。2.5~20μg/m L木脂素1处理Hep G2细胞后,G2/M期细胞所占百分比和细胞凋亡率显著增加(P值均<0.05),细胞中cleaved caspase 3蛋白表达水平显著上调(P<0.05)。结论 :木脂素1可抑制肝癌Hep G2细胞的增殖,并诱导其凋亡,这一作用可能与cleaved caspase 3蛋白的表达上调有关。
Objective: To investigate the effect of a novel compound lignan 1 on proliferation and apoptosis of human hepatocellular carcinoma Hep G2 cells. Methods: Hep G2 cells were treated with different concentrations of lignan 1. MTT assay was used to detect the proliferation of Hep G2 cells in each group. The cell cycle distribution and apoptosis of Hep G2 cells in each group were detected by FCM. Western blotting The expression level of cleaved caspase 3 protein in Hep G2 cells of each group. Results: Hep G2 cells were treated with lignan 1 at 1.25 ~ 20μg / ml for 24, 48 and 72 h. The proliferation of Hep G2 cells was significantly inhibited in a time-and dose-dependent manner (P <0.05). The percentage of cells in G2 / M phase and the apoptosis rate in Hep G2 cells treated with 2.5 ~ 20μg / mL Lignan 1 increased significantly (all P <0.05), and the expression of cleaved caspase 3 protein in Hep G2 cells significantly increased P <0.05). CONCLUSION: Lignans 1 can inhibit the proliferation and induce the apoptosis of Hep G2 cells, which may be related to the up-regulation of cleaved caspase 3 protein.