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目的:考察中药水煎液不同前处理方法对大孔树脂吸附分离效果的影响,确定其内在规律。方法:以红景天水煎液为对象,红景天苷含量为指标,分别采用一次醇沉、二次醇沉、加入沉淀剂、沉淀剂加醇沉、离心5种精制处理方法,考察对大孔树脂吸附效果的影响。结果:二次醇沉法及沉淀剂合并醇沉的方法对水煎液的精制效果最佳,同时对大孔树脂的分离效果也最佳。结论:中药水煎液使用不同的前处理方法对大孔吸附树脂分离效果具有显著影响。
OBJECTIVE: To investigate the effect of different pretreatment methods of decoction of traditional Chinese medicine on the adsorption and separation of macroporous resin, and to determine its internal rules. Methods: Salidroside was used as an index and Salidroside content was taken as an index. Alcohol precipitation, secondary alcohol precipitation, adding precipitant, precipitating agent plus alcohol precipitation and centrifugation were used to study the effects of salidroside, Hole resin adsorption effect. Results: The method of double alcohol precipitation and precipitant combined with alcohol precipitation optimized the decoction. Meanwhile, the separation of macroporous resin was the best. Conclusion: The different pretreatment methods of decoction of traditional Chinese medicine have a significant effect on the separation of macroporous resin.