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百日咳全菌体菌苗可诱导对多种抗原[如百日咳毒素(PT)、丝状血凝素(FHA)、69KDa外膜蛋白(69KDa OMP)、凝集原2和3(AGG 2和3)以及其他外膜蛋白]的抗体应答。在百日咳菌苗中,这些抗原成分的含量影响其免疫应答。 作者测定了丹麦当地4种百日咳菌株等量混合的百日咳全菌体菌苗中相应抗原成分的含量。菌苗经甲醛和硫柳汞灭活制成。每剂菌苗含16浊度单位(菌体数约为16×10~9)。从菌苗中提取各种抗原,用ELISA测定菌苗中各种抗原和脂多糖(LPS)含量。 应用2M尿素,在60℃加热30分钟,随后超声处理45秒是提取FHA、69KDa OMP、AGG2和3的最有效方法。提取PT的最佳方法是1M NaCl 4℃过夜,随后超声处理。
Pertussis pertussis bacterins can induce the induction of multiple antigens such as pertussis toxin (PT), filamentous hemagglutinin (FHA), 69 kDa outer membrane protein (69 kDa OMP), agglutinogens 2 and 3 (AGG 2 and 3) Other outer membrane proteins]. In pertussis vaccine, the content of these antigenic components affects their immune response. The authors measured the content of the corresponding antigenic components in whole-body pertussis bacteriophages mixed with equal amounts of four pertussis strains in Denmark. Vaccines are made of formaldehyde and thimerosal inactivated. Each vaccine contains 16 turbidity units (the number of cells is about 16 × 10 ~ 9). Various antigens were extracted from the bacterins, and the content of various antigens and lipopolysaccharide (LPS) in the vaccine was determined by ELISA. Applying 2M urea, heating at 60 ° C for 30 minutes followed by sonication for 45 seconds is the most efficient way to extract FHA, 69KDa OMP, AGG2 and 3. The best way to extract PT is 1 M NaCl overnight at 4 ° C followed by sonication.