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利用ISSR分子标记技术对福建省7个地区的茶树品种进行遗传多样性和遗传结构的分析。筛选出的12条引物共扩增出97条条带,其中多态性条带有92条,多态性谱带百分率为94.84%;53份供试材料可划分为5大类(GS=0.71),遗传距离介于0.66~0.99之间;Nei基因多样性指数为0.224,Shannon’s信息指数为0.368,不同地区茶树的遗传多样性参数差异较大,其中宁德福安地区最高,福州鼓山地区最低;总变异系数为0.332,地区内个体间遗传多样性为0.154,而地区间为0.231,福州地区与其他地区之间的相似性较弱;研究认为,福建省茶树遗传多样性丰富,不同地区茶树存在高频率遗传变异,而缺乏遗传信息交流是造成福州地区遗传多样性较低的可能因素。
ISSR molecular markers were used to analyze the genetic diversity and genetic structure of tea tree species in seven regions in Fujian Province. A total of 97 bands were screened out with 12 primers selected, of which 92 bands were polymorphic and 94.84% bands were polymorphic bands. The 53 tested cultivars could be divided into 5 groups (GS = 0.71 ), And the genetic distance ranged from 0.66 to 0.99. The Nei gene diversity index was 0.224 and the Shannon’s information index was 0.368. There were significant differences in genetic diversity among tea trees in different regions, with the highest in Fu’an and the lowest in Gushan, Fuzhou The coefficient of variation was 0.332. The genetic diversity among individuals in the region was 0.154, while the inter-region genetic distance was 0.231. The similarity between Fuzhou region and other regions was weak. According to the study, the tea tree in Fujian Province was rich in genetic diversity, Frequency of genetic variation, and lack of genetic information exchange is the genetic diversity of Fuzhou area may be lower.