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[目的]测定人胶质瘤细胞系A172,U87MG,U138,U373,和鼠胶质瘤细胞系GL261与MT539对细小病毒MVM和H1的易感性,并观察野生型病毒在肿瘤细胞内部繁殖情况。[方法]体外培养肿瘤细胞,分别用不同剂量的MVM和H1病毒感染GL261,MT539和A172,U87MG,U138,U373细胞1h,继续培养5d,每天进行活细胞计数。空斑形成试验测定感染病毒(MOI=0.1)5d后细胞内部及细胞培养上清中的病毒总量。[结果]与未感染细胞(对照)比较,U138,U373,GL261细胞在MOI2和5的病毒剂量感染后活细胞数目明显减少,U87细胞数基本维持不变,A172和MT539细胞数轻微增加。MOI=0.1的病毒剂量对细胞的作用与对照没有差异。空斑形成试验结果表明U373和GL261在MOI=0.1的病毒感染5d后细胞内和培养上清中总病毒量略高于初始病毒量,其它细胞的总病毒量均低于初始病毒量。[结论]不同胶质瘤对细小病毒的易感性不同,U138,U373,GL261细胞对细小病毒H1或MVM易感,U87细胞次之,A172和MT539细胞不敏感。U373和GL261细胞有弱的产生子病毒的能力。
[Objective] To investigate the susceptibility of human glioma cell lines A172, U87MG, U138, U373 and glioma cell lines GL261 and MT539 to parvoviruses MVM and H1 and to observe the propagation of wild type virus in tumor cells. [Methods] Tumor cells were cultured in vitro. GL261, MT539, A172, U87MG, U138 and U373 cells were infected with different doses of MVM and H1 virus respectively for 1 h and cultured for 5 days. The viable cells were counted daily. Plaque formation assay was used to determine the total amount of virus in the cells and in the cell culture supernatant after 5 days of infection (MOI = 0.1). [Results] The numbers of viable cells in U138, U373 and GL261 cells after virus dose infection at MOI 2 and 5 were significantly decreased compared with uninfected cells (control), the number of U87 cells remained basically unchanged, and the numbers of A172 and MT539 cells slightly increased. The effect of virus dose at MOI = 0.1 on cells did not differ from the control. The results of plaque formation assay showed that the total amount of virus in U373 and GL261 cells was slightly higher than that of the initial virus after 5 days of infection with MOI = 0.1. The total amount of virus in other cells was lower than the initial amount of virus. [Conclusion] Different gliomas have different susceptibility to parvovirus. U138, U373 and GL261 cells are susceptible to parvoviruses H1 or MVM, followed by U87 cells and not sensitive to A172 and MT539 cells. U373 and GL261 cells have a weak ability to produce a subvirus.