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目的研究不同浓度的2,3-丁二酮单肟(BDM)初始复灌对停搏心脏机械功能和氧代谢恢复的影响,确定理想作用浓度并初步探讨其机制。方法48只Langendorff灌流豚鼠心脏用4℃St.Thomas Hospital液保存12h,随机分为4组,分别以37℃改良Krebs-Ringer液(CON组),含20mmol/L BDM的Krebs液(BDM20组)、含30mmol/L BDM的Krebs液(BDM30组)、含40mmol/L BDM的Krebs液(BDM40组)进行初始复灌30min,随后所有心脏以37℃改良Krebs-Ringer液进行复灌60min。结果与CON组比较,BDM初始灌流后左室作用压、左室压力增加速率峰值(dP/dtmax)和左室压力减少速率峰值(-dP/dtmax)、氧消耗速度和用氧效率恢复更为迅速,BDM30组最为显著。电镜检查发现,CON组心肌呈明显的肌节过度收缩和线粒体破坏,BDM30组则明显减轻。结论BDM初始灌流可显著改善停搏心肌的机械功能和氧代谢,且理想作用浓度为30mmol/L,其机制可能与BDM减轻缺血-再灌注引起的心肌过度收缩和细胞破坏有关。
Objective To study the effects of initial reperfusion of 2,3-butanedione mono-oxime (BDM) on cardiac mechanical function and recovery of oxygen metabolism in cardiomyocytes. The optimal concentration of 2,3-dimethylglyoxime (BDM) was determined and its mechanism was explored. Methods 48 guinea pigs perfused with Langendorff were stored in St. Thomas Hospital fluid for 12 hours and divided into 4 groups at random. They were treated with 37 ℃ modified Krebs-Ringer solution (CON), 20 mmol / L BDM Krebs solution (BDM20) , Krebs solution (BDM30 group) containing 30 mmol / L BDM, Krebs solution (BDM40 group) containing 40 mmol / L BDM for 30 min, then all hearts were reperfused for 60 min with 37 ℃ modified Krebs-Ringer solution. Results Compared with CON group, left ventricular pressure, peak dp / dtmax and -dP / dtmax, oxygen consumption rate and oxygen efficiency recovery after BDM initial perfusion were more Quickly, BDM30 group the most significant. Electron microscopy showed that CON group showed obvious sarcolemma excessive contraction and mitochondrial damage, BDM30 group was significantly reduced. Conclusion Initial perfusion of BDM can significantly improve mechanical function and oxygen metabolism of myocardium, and the optimal concentration is 30mmol / L. The mechanism may be related to the effect of BDM in reducing myocardial over-contraction and cell destruction induced by ischemia-reperfusion.