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目的 探讨体内外镉的免疫功能抑制过程中促肾上腺皮质激素释放因子 (CRF)的修饰调节作用。方法 用垂体前叶细胞培养法测定中枢下丘脑和外周血浆CRF水平 ,用 [3 H TdR]掺入法分别检测体内、体外染毒时鼠脾T、B淋巴细胞增殖转化功能。结果 (1)整体实验动物各镉剂量组中枢下丘脑组织CRF水平均升高 ,有丝分裂原诱导脾T、B淋巴细胞增殖转化的刺激指数均低于对照组 ,差异均有显著性 (P <0 .0 5 ) ;下丘脑CRF水平与T、B淋巴细胞增殖指数之间呈负相关关系 (r =- 0 .6 75 0、- 0 .75 6 0 ,P <0 .0 5 ) ;外周血浆CRF水平各组间差异无显著性 (P >0 .0 5 )。 (2 )体外染毒时 ,镉明显抑制大鼠脾淋巴细胞的增殖转化 ;1.0～ 10 0 .0nmol/LCRF明显增强T、B淋巴细胞增殖转化能力 ;当以 1.0nmol/LCRF与 5～ 2 0 μmol/L氯化镉联合作用时各实验组两种免疫细胞 [3 H TdR]掺入率依然高于对照组 ,差异均有显著性 (P <0 .0 5 )。结论 体内染镉时存在中枢组织CRF介导的免疫功能抑制性调节作用 ,但体外CRF却能增强免疫细胞活性并阻断镉对鼠脾淋巴细胞的直接免疫功能抑制。CRF对镉的淋巴细胞免疫毒性具有双向调节作用。
Objective To investigate the regulatory effect of corticotropin-releasing factor (CRF) on the inhibition of cadmium immunosuppression in vivo and in vitro. Methods The level of CRF in the hypothalamus and peripheral blood of the central nervous system was measured by the method of anterior pituitary cell culture. The proliferation and transformation of T and B lymphocytes were detected by [3H] dR incorporation method in vitro and in vivo. Results (1) The CRF levels of central hypothalamus were increased in all cadmium groups. The mitotic index of spleen T and B lymphocyte proliferation and transformation were all lower than those in control group (P <0. .0 5). There was a negative correlation between CRF level in hypothalamus and proliferation index of T, B lymphocytes (r = - 0.7650, -0.756 0, P <0.05) There was no significant difference between the CRF levels (P> 0.05). (2) Cadmium significantly inhibited the proliferation and transformation of splenic lymphocytes in rats exposed to cadmium in vitro; 1.0 ~ 100 nmol / L CRF significantly enhanced the proliferation and transformation ability of T and B lymphocytes; when 1.0 nmol / L CRF and 5 ~ 20 The incorporation of [3 H TdR] in both experimental groups was still higher than that of the control group (P <0.05). Conclusions CRF-mediated suppression of central nervous system (CRF) -mediated immunosuppression exists in vivo when cadmium is absorbed in vivo. However, CRF in vitro enhances the activity of immune cells and blocks the direct immunosuppressive effect of cadmium on mouse splenic lymphocytes. CRF has a two-way regulatory effect on the immunotoxicity of cadmium to lymphocytes.