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目的建立有效的紫甘薯花色苷提取物分离纯化方法,为其产业化发展提供理论依据。方法通过静态吸附解吸试验筛选适合耐盐紫甘薯Z103花色苷纯化的大孔树脂,并进行动态吸附解吸工艺条件优化。结果在供试树脂中,HPD-300大孔树脂纯化效果最好。其静态吸附达平衡的时间为6~8 h,解吸附平衡时间为1~2 h,最大吸附量为33.67 mg·g-1。动态吸附解析最适工艺条件为:上样流速1.0 mL·min-1,上样浓度为0.3 mg·mL-1,体积为145 mL时,HPD-300树脂动态最大吸附量为13.82 mg·g-1(树脂),采用80%乙醇溶液作为洗脱剂,洗脱流速1.0 mL·min-1,用3倍柱体积洗脱花色苷,收率可达92.01%。纯化后的紫甘薯花色苷含量为181.58 mg·g-1,比纯化前提高13.18倍;色价1%1 cm E(528 nm)为142.63,比纯化前提高11.01倍。结论该工艺具有较好的实用性与参考价值。
Objective To establish an effective purification and purification method for anthocyanin extract of purple sweet potato and provide a theoretical basis for its industrialization. Methods The macroporous resin suitable for purification of purple sweet potato Z103 anthocyanins was screened by static adsorption and desorption test, and the conditions of dynamic adsorption and desorption were optimized. Results In the test resin, HPD-300 macroporous resin purification best. The static adsorption equilibrium time is 6 ~ 8 h, desorption equilibrium time is 1 ~ 2 h, the maximum adsorption capacity is 33.67 mg · g-1. The optimum conditions of dynamic adsorption analysis were as follows: the maximum dynamic adsorption capacity of HPD-300 resin was 1.0 mL · min-1 at the loading concentration of 0.3 mg · mL-1 and the volume of 145 mL was 13.82 mg · g- 1 (resin), using 80% ethanol as eluent, the elution flow rate was 1.0 mL · min-1, and the anthocyanin was eluted with 3 column volumes. The yield was 92.01%. Purified purple sweet potato anthocyanin content of 181.58 mg · g-1, 13.18 times higher than before purification; color value 1% 1 cm E (528 nm) of 142.63, 11.01 times higher than before purification. Conclusion The process has good practicality and reference value.