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目的 调查大深度饱和潜水时潜水员的应激反应。方法 用比色法检测潜水各阶段潜水员 2 4h尿 17-羟皮质类固醇 (17- OHCS)浓度 ;用荧光法分别检测尿儿茶酚胺 (CA)、肾上腺素 (A)和去甲肾上腺素 (NA)含量 ;用放免法测定潜水前后血浆内促肾上腺皮质激素 (ACTH)、促卵泡生成素 (FSH)、睾酮 (T)、生长激素 (GH)及皮质醇 (F)含量。结果 饱和暴露阶段 17- OHCS、CA、A、NA及出舱后ACTH、FSH、T、GH和 F水平均显著高于潜水前 (P<0 .0 5～ 0 .0 1)。结论 大深度现场饱和潜水期间能够引起潜水员的应激反应
Objective To investigate the stress response of divers during deep saturation diving. Methods The colorimetric method was used to detect the concentrations of urinary 17-OHCS in 24 hours of divers in different stages of diving. The contents of urinary catecholamine (CA), epinephrine (A) and norepinephrine (NA) The content of adrenocorticotropic hormone (ACTH), follicle stimulating hormone (FSH), testosterone (T), growth hormone (GH) and cortisol (F) in plasma before and after the diving were measured by radioimmunoassay. Results The levels of 17-OHCS, CA, A, NA and the levels of ACTH, FSH, T, GH and F at the saturated exposure stage were significantly higher than those before diving (P <0.05-0.01). CONCLUSIONS Deep-depth on-site saturation diving can cause divers’ stress response