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以典型粳籼交组合 (巴利拉×南京 1 1 ) F1 为试验材料 ,研究不同诱导培养基、分化培养基以及 ABT生根粉与其他植物激素配合使用对该组合进行花药培养的效应。结果表明 :源于含 2 ,4 -二氯苯氧乙酸 (2 ,4 -D)、α-萘乙酸 (NAA)、激动素 (KT)诱导培养基的愈伤组织 ,其绿苗分化率高于仅含 2 ,4 -D诱导培养基的愈伤组织 ;以 2 mg/L的 6-苄基氨基嘌呤 (6-BA)代替 KT组成的分化培养基可提高该组合的绿苗分化率 ;在 MS分化培养基中添加 5mg/L ABT生根粉可促进绿苗的分化。在 MS壮苗培养基中添加 5.0～ 7.5mg/L ABT生根粉可使再生植株生长矮壮繁茂 ,不定芽及次生根数目显著增加 ,并可提高再生植株移栽成活率。ABT生根粉、多效唑 (MET)、衣康酸衍生物 I(NCAI)以不同浓度配合使用 ,可用于调节试管苗根芽生长速度 ,以适应组织培养中不同时期对试管苗的不同要求
The effects of different induction medium, differentiation medium, ABT rooting powder and other plant hormones on the combination of anther culture were studied by using F1, a typical japonica rice combination (Barila × Nanjing 1 1) as test material. The results showed that callus derived from 2, 4 - dichlorophenoxyacetic acid (2, 4-D), α-NAA and KT inducing medium had high rate of green shoot differentiation In callus containing only 2, 4-D induction medium, the differentiation medium consisting of 6-benzylaminopurine (2-BA) at 2 mg / L instead of KT increased the green shoot differentiation rate of this combination. Addition of 5mg / L ABT rooting powder to MS differentiation medium can promote the differentiation of green shoots. The addition of 5.0-7.5mg / L ABT rooting powder to MS seedling growth medium can make the regenerated plants grow more and more vigorously, the number of adventitious buds and secondary roots are significantly increased, and the transplanting survival rate of regenerated plants can be increased. ABT rooting powder, MET and ITAI can be used in different concentrations to adjust the growth rate of root sprouts in vitro and in order to meet the different requirements of tube seedlings in different periods of tissue culture