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目的 观察异丙酚对培养海巴神经元缺氧复氧后损伤反应的影响。方法 培养 12d海巴神经元 ,随机分为正常对照组 (Nor组 )、缺氧 4h后复氧 2 4h组 (Ano组 ) ,加异丙酚 5 0 0 μmol/L缺氧 4h后复氧 2 4h组 (Pro组 )。MTT法测定细胞存活率及用流式细胞仪测定C fos含量和神经元凋亡率。结果 经流式细胞仪测定显示 ,缺氧后Ano组C fos蛋白表达的λm 值及细胞凋亡率明显增加 (P <0 .0 1) ,Pro组能减少阳性率的改变 ,λm 值和细胞凋亡率较Ano组减少 (P <0 .0 5 ) ,但与Nor组仍有差异 (P <0 .0 1)。MTT法测定的细胞存活率同凋亡的测定结果一致。结论 异丙酚能提高体外海马神经元的抗缺氧能力 ,减少C fos蛋白过度表达和细胞的凋亡。
Objective To observe the effect of propofol on hypoxic-reoxygenation injury in cultured hippocampal neurons. Methods Hippocampal neurons were cultured for 12 days. They were randomly divided into normal control group (Nor group), hypoxia group (Ano group) 4h after hypoxia, hypoxia 4h after propofol 500 micromol / L, reoxygenation 2 4h group (Pro group). Cell viability was measured by MTT assay and C fos content and neuronal apoptosis rate were determined by flow cytometry. Results The results of flow cytometry showed that the expression of C fos mRNA and the apoptosis rate of Ano group were significantly increased after hypoxia (P <0.01), while the Pro group decreased the positive rate, The apoptotic rate was lower than that of Ano group (P <0.05), but still different from that of Nor group (P <0.01). Cell viability assayed by MTT assay was consistent with the assay of apoptosis. Conclusion Propofol can increase hippocampal neurons against hypoxia in vitro and decrease C fos protein overexpression and cell apoptosis.