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目的:探讨中药合剂对种植体周围骨整合的作用机理;方法:健康雄性6m龄新西兰大白兔24只,随机分为对照组(12只)和实验组(12只)。实验组饲料中加入补肾中药合剂,对照组常规饲料。分别喂养3 d后在其胫骨顶端植入纯钛种植体3颗。于种植后30、60、90 d处死相同例数动物(n=6)。免疫组织化学技术检测OPG、PGL、COX-2的表达变化;结果:30 d时实验组OPG、OPGL、COX-2均中度阳性表达,明显高于对照组(弱阳性),两组间有统计学意义(P<0.05);60d时实验组OPG、OPGL表达(强度阳性)明显高于对照组(中强阳性),两组间有统计学意义(P<0.05),而两组COX-2均弱阳性表达,两组间没有统计学意义(P>0.05);90d两组OPG、OPGL、COX-2均弱阳性表达,两组间没有统计学意义(P>0.05);结论:OPG、OPGL和COX-2参与了种植后的骨组织修复过程,而中药合剂能进一步提高种植术后OPG、OPGL和COX-2的表达水平,提示补肾中药合剂可能促进种植体的周围骨整合。
Objective: To explore the mechanism of traditional Chinese medicine (TCM) mixture on the osseointegration of implant. Methods: Twenty-four healthy male 6-month-old New Zealand white rabbits were randomly divided into control group (12 rats) and experimental group (12 rats). In the experimental group, Bushen Chinese traditional medicine was added into the feedstuff, and the control group was given routine feed. Three days after feeding, three pure titanium implants were implanted in the tip of the tibia. The same number of animals (n = 6) were sacrificed 30, 60 and 90 days after planting. The expression of OPG, PGL and COX-2 were detected by immunohistochemistry. Results: At 30 days, the expression of OPG, OPGL and COX-2 in the experimental group were significantly higher than those in the control group (weakly positive) (P <0.05). At 60 days, the expression of OPG and OPGL in the experimental group was significantly higher than that in the control group (moderately strong positive), with statistical significance (P <0.05) (P> 0.05). The expression of OPG, OPGL and COX-2 in both groups were weakly positive at the 90th day, and there was no statistical significance between the two groups (P> 0.05) .Conclusion: OPG , OPGL and COX-2 were involved in the repair process of bone tissue after implantation. However, traditional Chinese medicine mixture can further improve the expression of OPG, OPGL and COX-2 after implantation, suggesting that tonifying kidney preparations may promote the osseointegration of implants.