1.取普通滤纸用打孔机制成直径6毫米大小的圆形小片,每100片封装于一洁净的青霉素小瓶中,高压灭菌15磅30分钟。2.先把各种抗菌素溶液用生理盐水配成下列浓度的溶液:磺胺类药物100毫克/毫升。青霉素1,000单位/毫升,其他各种抗菌素(链霉素、氯霉素、金霉素、新霉素、红霉素、卡那霉素、杆菌肽、呋喃西林等)10毫克/毫升。3.在已高压灭菌的装有滤纸片的小瓶内分别加丙酮1毫升,再各加上述浓度抗菌素溶液0.1毫升,充分混合均匀,在室温浸泡2小时。4.将浸泡后的滤纸片和抗菌素溶液一起倒到无 1. Take ordinary filter paper with a punch made of 6 mm in diameter round small pieces, each 100 packed in a clean penicillin vials, autoclaved 15 pounds for 30 minutes. 2. The first solution of various antibiotics with physiological saline dubbed the following concentrations of the solution: sulfa drugs 100 mg / ml. Penicillin 1,000 units / ml, other various antibiotics (streptomycin, chloramphenicol, chlortetracycline, neomycin, erythromycin, kanamycin, bacitracin, nitrofurazone, etc.) 10 mg / ml. 3. In autoclaved filter paper vials were added acetone 1 ml, then add the above concentration 0.1 ml of antibiotic solution, thoroughly mixed, soaked for 2 hours at room temperature. 4. The soaked filter paper and antibiotic solution together to no