论文部分内容阅读
生肌调节因子是肌肉形成的关键控制因素.通过RT-PCR方法克隆得到鳜鱼主要生肌调节因子MyoD序列.鳜鱼MyoD基因cDNA长度为813 bp,编码270个氨基酸,推导的氨基酸序列含有1个bHLH结构.经与其他动物MyoD氨基酸序列比对发现不同动物MyoD的bHLH结构域保守性高.通过合成地高辛标记的反义RNA探针对鳜鱼不同发育阶段的胚胎进行整体胚胎原位杂交,结果发现:MyoD最早在肌节早期被检测到信号.伴随着鳜鱼体节从头部往后发育,MyoD也从头部往尾部表达.在体节期,MyoD在中后部表达.尾芽期MyoD的表达量达到最大值.然而,尾芽期过后,头部MyoD的表达量急剧减少,但尾部仍然有强烈的信号.在血流期仍然能在鳜鱼尾部检测到MyoD的微弱信号.到孵出期在鳜鱼鱼眼的后方和胸部腹侧检测到MyoD的强信号,而身体其他地方检测不到信号.仔鱼期没有检测到MyoD表达的信号.
Myogenic regulatory factor is the key controlling factor of muscle formation.MyoD sequence of major myogenic regulator of anchovy was obtained by RT-PCR method.The length of MyoD gene was 813 bp, encoding 270 amino acids and the deduced amino acid sequence contained 1 A bHLH structure.The amino acid sequence comparison of MyoD with other animals showed that the bHLH domain of different animal MyoD was highly conserved.Through the synthesis of digoxigenin labeled antisense RNA probe, The results showed that MyoD was detected as early as the sarcomere, and MyoD was also expressed from the head to the tail with the development of the bonito section from the head to the tail. However, the expression of MyoD in the head decreased sharply after tail bud, but there was still a strong signal in the tail, and the weak MyoD was still detected in the tail of the anchovy in the blood flow Signal to MyoD at the rear of hatchling and chest ventral to hatching, whereas no signal was detected elsewhere in the body. No signal was detected for MyoD expression in larval stage.