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目的研究小鼠经中子及γ射线照射后肠组织中EGF和EGFR的表达变化及其意义。方法350只二级雄性BALB/C小鼠,经不同剂量的中子和γ射线照射,于照后6h、12h及1、2、3、4、5、7、10、14、21、28天分批活杀,采用免疫组化法研究EGF和EG-FR在肠组织中的变化。结果肠黏膜上皮和隐窝细胞浆内EGF及EGFR表达在2·5Gy中子照后1天内增强,1~2天减少,3~7天增多,5天达高峰,14天后恢复至正常水平;4·0、5·5Gy中子及12Gyγ射线照射后6h增多,12h~4天进行性减少;5·5Gyγ射线照射后3天内增加,3天达高峰,5天后逐渐恢复。结论中子和γ射线照射后肠内源性EGF及EGFR的表达具有不同的变化规律,参与了肠放射损伤及修复的病理过程。
Objective To study the changes and significance of EGF and EGFR in the intestinal mucosa of mice irradiated by neutron and γ-rays. Methods 350 male BALB / C male mice were irradiated with different doses of neutron and γ-ray. After 6h, 12h and 1,2,3,4,5,7,10,14,21 and 28 days The mice were sacrificed in batches and the changes of EGF and EG-FR in intestinal tissues were studied by immunohistochemistry. Results The expression of EGF and EGFR in intestinal epithelial cells and crypt crypts increased within 1 day after 2.5 Gy neutrophils, decreased in 1 to 2 days, increased in 3 to 7 days, peaked in 5 days and returned to normal levels in 14 days. 4 · 0,5 · 5Gy neutrons and 12Gy γ rays increased 6h after irradiation, 12h ~ 4 days progressive decrease; 5 · 5Gy γ-ray increased within 3 days after 3 days reached its peak, gradually recovered after 5 days. Conclusions The expressions of endogenous EGF and EGFR in the gut after neutron and gamma irradiation have different rules, which are involved in the pathological process of intestinal radiation injury and repair.