单纯疱疹病毒胸腺嘧啶核苷酸酶（ＨＳＶｔｋ）基因是一种自杀基因，其产物可将无毒的核苷类似物如Ｇａｎｃｉｌｏｖｉｒ（ＧＣＶ）转变成有毒的产物，从而将细胞杀死〔１〕。近年来，许多学者利用多种载体将ＨＳＶｔｋ基因转入肿瘤细胞。腺病毒是继逆转录病毒之后一种非常 The herpes simplex virus thymidine kinase (HSV-tk) gene is a suicide gene whose product converts nontoxic nucleoside analogs such as Gancilovir (GCV) into toxic products, killing the cell [1 ]. In recent years, many scholars use a variety of vectors to transfer HSV  tk gene into tumor cells. Adenoviruses are the second most common form of retroviruses