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目的:通过研究二仙汤对卵巢血供和卵泡颗粒细胞分泌AMH、Inhibin的影响,探究其对卵巢储备功能的影响,从而探究二仙汤治疗卵巢早衰的机制。方法:60只SD大鼠随机分为正常组、模型组、阳性对照组和二仙汤组,腹腔注射顺铂建立化疗性损伤大鼠卵巢早衰动物模型。造模后连续4周灌胃药物治疗。HE染色观察卵巢血管的变化;ELISA法测定血清中AMH、Inhibin含量。结果:与正常组比较,模型组卵巢血管减少,血清AMH、Inhibin下降(P<0.05);二仙汤和西药补佳乐治疗后与模型组比较卵巢血管增多,血清AMH、Inhibin升高(P<0.05)。结论:二仙汤治疗卵巢早衰的机制之一可能是改善卵巢血供,促进颗粒细胞AMH和Inhibin分泌,恢复卵泡正常募集和成熟,减少卵泡闭锁,从而改善卵巢储备功能。
Objective: To study the effect of Erxian Decoction on the secretion of AMH and Inhibin by ovarian blood supply and follicular granulosa cells, and to explore the effect of Erxian decoction on ovarian reserve function, so as to explore the mechanism of Erxian decoction in treating premature ovarian failure. Methods: Sixty Sprague Dawley rats were randomly divided into normal group, model group, positive control group and Erxian decoction group. The animal model of premature ovarian failure was established by intraperitoneal injection of cisplatin. After 4 weeks of continuous modeling drug treatment. The changes of ovarian blood vessels were observed by HE staining. The contents of AMH and Inhibin in serum were determined by ELISA. Results: Compared with the normal group, the ovarian blood vessels in the model group decreased, while the levels of serum AMH and Inhibin decreased (P <0.05). Compared with the model group, Erxian decoction and western medicine did not increase the level of serum AMH and Inhibin (P <0.05). Conclusion: One of the mechanisms of Erxian Decoction in treating premature ovarian failure may be to improve the blood supply of ovaries, promote the secretion of AMH and Inhibin in granulosa cells, restore normal follicular recruitment and maturation, and reduce the follicular atresia so as to improve ovarian reserve function.