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以金线吊乌龟的幼嫩茎段为材料,经消毒处理后,剪成带一个腋芽的茎段,在1/2 MS+5μmol/L KT+0.5μmol/L NAA培养基上进行培养,获得无菌芽苗,再以无菌苗的单芽体为外植体,建立了金线吊乌龟的组培快繁体系。结果表明:最佳丛生芽增殖培养基为1/2 MS+10μmol/L KT+0.5μmol/L NAA+20μmol/L CCC,30 d的增殖系数为4.83;芽苗生根的最适培养基为1/4 MS+10μmol/L NAA;炼苗后,移入泥炭土基质中,成活率达95.0%。
The young stem of the golden turtle was used as material to sterilize and then cut into one axillary bud stem section and cultured on 1/2 MS +5 μmol / L KT + 0.5 μmol / L NAA medium to obtain Aseptic buds, and then a single shoot buds of sterile seedlings as explants, the establishment of gold wire turtles tissue culture rapid propagation system. The results showed that the optimum culture medium was 1/2 MS + 10μmol / L KT + 0.5μmol / L NAA + 20μmol / L CCC, and the multiplication coefficient was 4.83 on the 30th day. The optimum culture medium for rooting was 1 / 4 MS + 10μmol / L NAA. After the seedling was made into the peat soil matrix, the survival rate was 95.0%.