论文部分内容阅读
目的建立白喉类毒素双抗体夹心ELISA检测方法,并进行验证及初步应用。方法以马抗白喉类毒素血清作为包被抗体,HRP标记的白喉絮状反应抗毒素作为酶标抗体,建立白喉类毒素双抗体夹心ELISA检测方法,并对该方法进行重复性、特异性验证、最佳线性范围和检测限确定及初步应用。结果经验证,该方法重复性好,特异性强,白喉类毒素含量在0.976 5～125.000 0 ng/ml之间时线性关系良好,检测限为7.812 ng/ml,该方法检测了3批白喉类毒素原液,与动物实验结果基本相符。结论已建立了白喉类毒素双抗体夹心ELISA检测方法,可用于检测白喉类毒素的含量和抗原性。
Objective To establish a diphtheria toxoid double antibody sandwich ELISA detection method, and to verify and preliminary application. Methods The anti-diphtheria toxoid serum was used as coated antibody and HRP-labeled diphtheria flocculent anti-toxin as enzyme-labeled antibody to establish the diphtheria toxoid double-antibody sandwich ELISA test method. The method was repeated and specific verification Good linear range and limit of detection and the initial application. Results The method was proved to be reproducible and specific. The linearity of diphtheria toxoid was between 0.976 5 and 125.000 0 ng / ml with a detection limit of 7.812 ng / ml. The method was used to detect three batches of diphtheria Toxin solution, and animal experiments basically consistent with the results. Conclusion Diphtheria toxoid double antibody sandwich ELISA assay has been established and can be used to detect diphtheria toxoid content and antigenicity.