论文部分内容阅读
目的 研究三乙酰莽草酸 (TSA)对局灶性脑缺血后脑组织损伤保护作用及对血液流变学变化的影响。方法 线栓塞法阻断大脑中动脉造成大鼠局灶性脑缺血。TTC染色法测定脑梗死范围。测定脑缺血 2 4h后红细胞变形和集聚能力、全血和血浆粘度。结果 脑缺血 2 4h后 ,脑梗死范围达前脑的 ( 16 .6± 3 .6 ) % ,对侧半球的 ( 32 .0± 8.9) %。脑缺血后立即和 6 0min 2次灌胃给TSA 2 5 ,5 0和 10 0mg·kg- 1 ,可使脑梗死范围 (按占对侧半球的百分比 )分别减少 9.6 % ,2 1.7% (P <0 .0 1)和 31.9% (P <0 .0 1)。TSA 5 0和 10 0mg·kg- 1 还可降低大鼠的神经功能行为评分。尼莫地平 5mg·kg- 1 也可减少脑梗死范围 ,降低神经功能行为评分。脑缺血 2 4h后红细胞变形能力下降 ,红细胞聚集程度、血粘度及血浆粘度皆明显提高。TSA 10 0mg·kg- 1 可明显降低红细胞聚集程度 ,但对红细胞变形和血粘度及血浆粘度无显著影响。尼莫地平可降低切变率为 2 0 0～ 1s- 1 范围内的全血粘度及血浆粘度。结论 TSA对局灶性脑缺血引起的脑损伤有一定的保护作用
Objective To investigate the protective effect of triacetylshikimic acid (TSA) on brain injury following focal cerebral ischemia and its effect on the changes of hemorrheology. Methods The occlusion of middle cerebral artery occlusion caused by focal embolization in rats resulted in focal cerebral ischemia. TTC staining to determine the extent of cerebral infarction. After 24 hours of cerebral ischemia, erythrocyte deformability and aggregation ability, whole blood and plasma viscosity were measured. Results After 24 h of cerebral ischemia, the range of cerebral infarction was (16.6 ± 3.66)% in the forebrain and (32.0 ± 8.9)% in the contralateral hemisphere. Cerebral infarction range (percentage of the contralateral hemisphere) was reduced by 9.6% and 2 1.7% (P <0.05), respectively, to TSA 25, 50 and 100 mg · kg -1 immediately after cerebral ischemia and 60 min P <0. 01) and 31.9% (P <0. 01). TSA 50 and 10 0 mg · kg -1 also reduced the neurological function score in rats. Nimodipine 5mg · kg-1 can also reduce the range of cerebral infarction, reduce neurological function score. After 24 hours of cerebral ischemia, erythrocyte deformability decreased, erythrocyte aggregation, blood viscosity and plasma viscosity were significantly increased. TSA 10 0 mg · kg-1 can significantly reduce the degree of erythrocyte aggregation, but no significant effect on erythrocyte deformation and blood viscosity and plasma viscosity. Nimodipine can reduce whole blood viscosity and plasma viscosity in the range of shear rate of 200 ~ 1s-1. Conclusion TSA can protect brain injury induced by focal cerebral ischemia