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目的:探讨经乙型肝炎病毒(HBV)转基因小鼠树突状细胞(DCs)体外诱导的特异性免疫效应细胞(IECs)对自体HBV复制的影响。方法:从转HBV基因小鼠体内提取DCs,体外诱导为成熟DCs,与淋巴细胞共培养,诱导为特异性IECs,将其经阴茎背静脉注入小鼠体内。实验分为2组:生理盐水(NS)组、IEC组,观察6个时间点:0 h、2、4、6、8周和12周;通过生化检测肝功能,PCR检测血清中HBV DNA水平、ELISA检测细胞因子、免疫组化检测肝组织内的HBs Ag和HBc Ag,评估IECs对小鼠体内HBV复制的影响。结果:IEC组小鼠于6、8周和12周时,肝功能明显改善,HBV DNA水平明显降低,HBs Ag和HBc Ag明显减少,且均优于NS组(P<0.05)。结论:特异性IECs可修复转HBV基因小鼠肝脏功能、抑制HBV复制,相关的细胞因子参与其作用。
Objective: To investigate the effects of specific immune effector cells (IECs) induced by dendritic cells (DCs) of Hepatitis B virus (HBV) transgenic mice on their own HBV replication. METHODS: DCs were isolated from mice transfused with HBV and induced to mature DCs in vitro. The DCs were co-cultured with lymphocytes and induced into specific IECs. The DCs were injected into the mice via the dorsal penile vein. The experiment was divided into two groups: NS group, IEC group, 6 time points were observed: 0 h, 2, 4, 6, 8 and 12 weeks; serum HBV DNA level was detected by biochemical detection of liver function , Cytokines were detected by ELISA, HBsAg and HBcAg in liver tissue were detected by immunohistochemistry, and the effect of IECs on HBV replication in mice was evaluated. Results: At 6, 8 and 12 weeks, the liver function of IEC mice was significantly improved, and the level of HBV DNA was significantly decreased. The HBs Ag and HBc Ag levels were significantly lower than those of NS group (P <0.05). CONCLUSIONS: Specific IECs can repair the liver function of HBV transgenic mice and inhibit the HBV replication, and the related cytokines participate in its function.