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目的 探讨IL- 8、IL- 10在大鼠胰腺炎肺损伤中的作用。方法 采用胰胆管逆行注射3.5%牛磺胆酸钠的方法,制作大鼠重症急性胰腺炎并发肺损伤模型。分别对胰腺损伤、肺损伤程度进行病理评分,测定肺组织湿/干质量比;ELISA法测定大鼠血清IL- 8、IL -10水平。结果 造模后1 h,大鼠即出现较明显的胰腺损伤,随着时间的延长,胰腺损伤逐渐加重,以12 h为最。造模后1 h,即出现肺损伤,光镜下表现为白细胞浸润、肺泡间隔增厚、肺水肿及肺泡腔萎陷;至4 h肺组织湿/干质量比开始增加,此后,肺损伤程度逐渐增加,以24 h为最。造模后1 h,大鼠血清IL- 8、IL- 10水平逐渐升高,至24h达最高。结论 IL -8、IL- 10参与了大鼠胰腺炎肺损伤的病理过程,胰腺炎肺损伤的过程可能是体内全身性炎症反应综合征(SIRS)和代偿性抗炎症反应综合征(CARS)失衡的结果。
Objective To investigate the role of IL-8 and IL-10 in lung injury of rat pancreatitis. Methods The pancreaticobiliary duct was injected retrogradely with 3.5% sodium taurocholate to make a rat model of severe acute pancreatitis complicated by lung injury. The pathological score of pancreatic injury and lung injury were respectively determined, and the wet / dry mass ratio of lung tissue was measured. The serum levels of IL-8 and IL-10 were measured by ELISA. Results 1 h after modeling, the rats showed obvious pancreatic injury, with the extension of time, the pancreatic injury gradually aggravated, with 12 h as the most. One hour after model establishment, lung injury occurred. The leukocyte infiltration, thickening of alveolar septa, pulmonary edema and alveolar collapse were observed under light microscope. The wet / dry mass ratio of lung tissue began to increase after 4 hours, and the degree of lung injury Gradually increase to 24 h for the most. At 1 h after model establishment, the levels of IL-8 and IL-10 in serum increased gradually and reached the highest level at 24 h. Conclusion IL-8 and IL-10 are involved in the pathological process of lung injury induced by pancreatitis in rats. The process of lung injury in pancreatitis may be systemic inflammatory response syndrome (SIRS) and compensatory anti-inflammatory response syndrome (CARS) Unbalanced result.