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目的探讨多药耐药基因、多药耐药相关蛋白(MRP)、肺耐药蛋白(LRP)、谷胱甘肽-S转移酶π(GST-π)mRNA在非小细胞肺癌(NSCLC)中的共表达情况及临床意义。方法1996-10~2001-10采用逆转录-多聚酶链反应技术(RT-PCR)检测哈尔滨医科大学呼吸内科49例NSCLC组织中上述耐药基因的4种多药耐药基因共表达水平,随访生存时间,计算生存概率。结果在NSCLC中总阳性率分别为53.1%,65.3%,67.3%,83.3%。两种和(或)两种以上阳性共表达率与单一阳性表达率之间有显著差异性(P<0.05)。阳性表达率在NSCLC的不同临床分期、分化程度间无差异性(P>0.05)。随着多药耐药基因共表达种类增加,生存曲线左移,生存时间缩短。结论NSCLC的MDR现象是由多药耐药基因共表达引起的,多药耐药基因共表达种类对预后有直接影响,可作为评价预后的可靠指标。
Objective To investigate the expression of multidrug resistance gene, multidrug resistance-related protein (MRP), lung resistance protein (LRP) and glutathione S-transferase π (GST-π) mRNA in non-small cell lung cancer Of co-expression and clinical significance. Methods The co-expression levels of four multidrug resistance genes in 49 NSCLC tissues of Harbin Medical University from 1996 to 2001 were detected by reverse transcription polymerase chain reaction (RT-PCR) Time, calculate the probability of survival. Results The total positive rates in NSCLC were 53.1%, 65.3%, 67.3% and 83.3%, respectively. There was a significant difference between two or more positive co-expression rates and single positive expression rates (P <0.05). The positive rate of NSCLC in different clinical stages, no difference between the degree of differentiation (P> 0.05). With the multi-drug resistance gene co-expression types increased, the survival curve to the left, shorter survival time. Conclusion The MDR phenomenon in NSCLC is caused by the co-expression of multidrug resistance genes. The coexpression of multidrug resistance genes has a direct effect on prognosis, which can be used as a reliable indicator of prognosis.