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目的 :探讨空肠弯曲菌 (Campylobacterjejuni,Cj)感染与Guillain Barr啨综合征 (GBS)的关系 ,以及Cj感染与抗 GM1抗体的关系 ;并进一步研究GBS患者人类白细胞抗原 (Humanleukocyteantigen ,HLA)和肿瘤坏死因子 (tumornecrosisfactor,TNF)基因的多态性。方法 :用ELISA方法检测 80例GBS患者血清中Cj的IgG、IgM、IgA抗体及抗 GM1抗体 ,同时从患者的大便中培养Cj;用改良的PCR 限制性片段长度多态性 (PCR RFLP)结合组间特异性引物法分别测定HLA DRB1和 DQB1基因的多态性 ;用PCR和微卫星方法测定TNFα等位基因的多态性。结果 :GBS患者Cj感染率为 5 1.3% ,抗 GM1抗体检出率为 42 .5 % ;近期Cj感染者抗 GM1抗体检出率 ( 6 3.4% )显著高于未感染者 ( 2 0 .5 % ) (P <0 .0 0 1) ;GBS与HLA DR及 DQ等位基因无明显相关性 (P >0 .0 5 ) ,而与TNFα2等位基因有显著相关性 (P <0 .0 5 )。结论 :Cj感染是GBS的重要诱发因素之一 ,血清中抗 GM1抗体的存在与近期Cj感染有关 ;TNFα2等位基因可能是GBS的易感基因。
Objective: To investigate the relationship between Campylobacterjejuni (Cj) infection and Guillain Barr’s syndrome (GBS) and the relationship between Cj infection and anti-GM1 antibody. To study the relationship between human leukocyte antigen (HLA) and tumor necrosis Tumor necrosis factor (TNF) gene polymorphism. Methods: Serum Cj IgG, IgM, IgA and anti-GM1 antibodies in 80 patients with GBS were detected by ELISA. Cj was also cultured from the patients’ stools. Combined with PCR RFLP The polymorphisms of HLA DRB1 and DQB1 genes were determined by group-specific primers. The polymorphisms of TNFα alleles were determined by PCR and microsatellite markers. Results: The prevalence of Cj infection was 53.3% in GBS patients and 42.5% in anti-GM1 antibodies. The detection rate of anti-GM1 antibody in Cj patients was significantly higher than that in non-infected patients (2.5%) %) (P <0.01). There was no significant correlation between GBS and HLA DR and DQ alleles (P> 0.05), but significant correlation with TNFα2 allele (P <0. 0) 5). Conclusion: Cj infection is one of the important inducing factors of GBS. The presence of anti-GM1 antibody in serum is related to the recent Cj infection. The TNFα2 allele may be a susceptible gene to GBS.