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目的:探讨肿瘤坏死因子α(tumor necrosis factor,TNF-α)和可溶性肿瘤坏死因子受体(soluble TNFRI,sTNFRI)对体外培养的子宫内膜异位症(Endometriosis)患者在位内膜基质细胞的影响,为子宫内膜异位症的生物学治疗提供新思路。方法:对EMS患者的在位子宫内膜基质细胞进行体外培养,分别用不同浓度的TNF-α(0、0.1、1.0、10.0和100.0ng/ml)或者同一浓度TNF-α(1ng/ml)培养不同时间(4、8、12、24、48和72h)或者TNF-α(1ng/ml)和sTNFRⅠ(2#g/ml)对基质细胞进行刺激并收集细胞上清液,利用双抗体夹心ELISA检测细胞培养上清液中IL-6、基质金属蛋白酶-3(matrix metalloproteinases,MMP-3)的水平。结果:TNF-α可促进体外培养的EMS子宫内膜基质细胞IL-6、MMP-3的分泌,而且IL-6、MMP-3浓度与TNF-α之间存在剂量、时间依赖关系(P<0.05),而sTNFRⅠ具有抑制TNF-α的作用。结论:TNF-α在EMS的发病机制中发挥重要的作用,其对EMS患者子宫内膜基质细胞的促黏附、侵袭、增殖等作用可能是通过促进IL-6、MMP-3的分泌来完成的;sTNFRⅠ有望用于EMS生物学治疗。
Objective: To investigate the effect of tumor necrosis factor (TNF-α) and soluble TNFRI (sTNFRI) on endometrial stromal cells in endometriosis Influence, provide new ideas for the biological treatment of endometriosis. Methods: The eutopic endometrial stromal cells of EMS patients were cultured in vitro and treated with different concentrations of TNF-α (0, 0.1, 1.0, 10.0 and 100.0 ng / ml) or the same concentration of TNF- The stromal cells were stimulated at different time (4, 8, 12, 24, 48 and 72 hours) or TNF-α (1ng / ml) and sTNFRI (2 # g / ml) The levels of IL-6 and matrix metalloproteinases-3 (MMP-3) in cell culture supernatants were detected by ELISA. Results: TNF-α could promote the secretion of IL-6 and MMP-3 in EMS endometrial stromal cells cultured in vitro, and there was a dose-dependent relationship between the concentrations of IL-6 and MMP-3 and TNF- 0.05), while sTNFR Ⅰ has the effect of inhibiting TNF-α. Conclusion: TNF-α plays an important role in the pathogenesis of EMS, which may contribute to the promotion of adhesion, invasion and proliferation of endometrial stromal cells in EMS patients by promoting the secretion of IL-6 and MMP-3 ; sTNFRI is expected to be used in EMS biological therapy.