论文部分内容阅读
目的探讨成釉细胞蛋白(AMBN)基因多态性与环境因素在燃煤型地方性氟中毒中的交互作用。方法采用1∶1病例对照研究方法,选取重庆市巫山县笃坪乡和奉节县兴隆镇2个氟病区人群作为观察对象:病例组121例、病区对照121人,并利用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)法对AMBN基因多态性进行分析。应用条件logistic回归模型分析重庆市人群中氟中毒发生的环境及遗传因素,根据交互作用指标判断该基因与环境的交互作用。结果抽样地区人群的尿氟水平(F)≥0.95 mg/L及AMBN基因的7号外显子(538_540delGGA)的-/-(GGA完全缺失)基因型与13号外显子(986C>T)的CT基因型是该人群氟中毒的危险因素,两者分别存在交互作用(OR=5.600,γ=1.543;OR=4.375,γ=1.296)。结论携带(538_540delGGA)-/-基因型、(986C>T)CT基因型与尿氟含量(F≥0.95 mg/L)在燃煤型氟中毒中存在一定交互作用,燃煤型氟中毒与遗传及环境因素交互作用有关。
Objective To investigate the interaction between polymorphism of ameloblast protein (AMBN) gene and environmental factors in coal-fired endemic fluorosis. Methods A 1: 1 case-control study was conducted in two Acute Fluorosis Populations in Ailing Township, Wushan County, Chongqing Municipality, and Xinglong Town, Fengjie County: 121 cases in the case group and 121 controls in the ward. Polymerase chain reaction Analysis of AMBN Gene Polymorphism by Restriction Fragment Length Polymorphism (PCR-RFLP). The conditional logistic regression model was used to analyze the environmental and genetic factors of fluorosis in Chongqing population. The interaction between this gene and the environment was judged according to the interaction index. Results The urinary fluoride level (F) ≥0.95 mg / L and the - / - (GGA complete deletion) of exon 7 (538_540delGGA) of AMBN gene were significantly different from those of CTL exon 13 (986C> T) Genotypes were the risk factors of fluorosis in this population. There was interaction between them (OR = 5.600, γ = 1.543; OR = 4.375, γ = 1.296). Conclusion There is a certain interaction between CT genotype of (538_540delGGA) - / -, genotype of (986C> T) and urinary fluoride (F≥0.95 mg / L) in coal combustion type fluorosis. And environmental factors related to the interaction.