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我们用ELISA法测定正常人与病人血清nDNA抗体水平,结果如下: 一、材料:①小牛胸腺DNA:工作浓度1mg/ml,用前以碳酸盐包被液1:50稀释。②酶标记羊抗人IgG结合物。③1%鱼精蛋白溶液。④标本:正常人血清采自本校体检学生。病人血清采自本校附院及济南市
We use ELISA method to determine normal and patient serum nDNA antibody levels, the results are as follows: First, the material: ① calf thymus DNA: working concentration 1mg / ml, before use carbonate coating solution 1:50 dilution. ② enzyme labeled goat anti-human IgG conjugate. ③ 1% protamine solution. ④ specimens: normal serum collected from our school students. Patient serum was collected from the Affiliated Hospital of Jinan University and Jinan City