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目的:探讨CD40和COX-2在食管鳞状细胞癌组织中的表达情况、临床病理意义及其与肿瘤血管生成的关系。方法:采用免疫组织化学方法研究79例食管癌和28例正常食管黏膜上皮组织中CD40和COX-2的表达情况,根据CD34表达计算食管癌组织平均微血管密度(MVD);分析CD40、COX-2的表达与食管癌发生部位、肿瘤大小、分化程度、MVD及淋巴结转移的关系。同时采用免疫细胞化学染色及Western Blot方法检测食管癌Eca109细胞和原代培养的正常食管上皮细胞CD40和COX-2在蛋白水平上表达的差异。结果:免疫组化结果表明,食管癌组织中CD40和COX-2的阳性表达率均明显高于正常食管鳞状上皮(54.43%vs10.71%,69.62%vs17.86%,P均<0.05)。CD40在食管癌中的表达与淋巴结转移有关,伴有淋巴结转移者CD40阳性表达率明显高于无淋巴结转移者(70.37%vs46.15%,P<0.05),但CD40表达与其他临床病理特征无明显相关。COX-2在食管癌中的阳性表达与肿瘤大小、部位、分化程度、有无淋巴结转移等均无明显相关(P>0.05)。CD40与COX-2在食管癌组织中表达明显相关(P<0.05),相关系数(Φ)为0.446。食管癌组织中MVD明显高于正常食管组织(25.02±5.52vs12.09±4.55,P<0.05)。CD40和COX-2阳性表达组食管癌组织MVD明显高于阴性表达组(26.37±6.02vs22.58±5.25,P<0.05)。体外研究结果表明,食管癌Eca109细胞CD40和COX-2表达均高于正常食管上皮细胞(P<0.05)。结论:CD40表达与食管鳞状细胞癌的发生、发展密切相关,CD40可能通过影响COX-2的表达促进食管癌组织微血管生成。
Objective: To investigate the expression of CD40 and COX-2 in esophageal squamous cell carcinoma and its clinicopathological significance and its relationship with tumor angiogenesis. Methods: The expressions of CD40 and COX-2 in 79 cases of esophageal carcinoma and 28 cases of normal esophageal epithelium were detected by immunohistochemical method. The mean microvessel density (MVD) of esophageal carcinoma was calculated according to the expression of CD34. The expressions of CD40, COX-2 The relationship between the expression of esophageal carcinoma and tumor site, tumor size, differentiation, MVD and lymph node metastasis. At the same time, immunocytochemical staining and Western Blot were used to detect the differences of protein expression between CD40 and COX-2 in esophageal cancer Eca109 cells and normal esophageal epithelial cells. Results: Immunohistochemistry showed that the positive rates of CD40 and COX-2 in esophageal cancer tissues were significantly higher than those in normal esophageal squamous epithelium (54.43% vs10.71%, 69.62% vs17.86%, P <0.05, respectively) . The expression of CD40 in esophageal cancer was correlated with lymph node metastasis. The positive rate of CD40 expression in patients with lymph node metastasis was significantly higher than that without lymph node metastasis (70.37% vs46.15%, P <0.05), but there was no correlation between the expression of CD40 and other clinicopathological features Obviously related. COX-2 positive expression in esophageal cancer and tumor size, location, degree of differentiation, with or without lymph node metastasis were not significantly correlated (P> 0.05). The expressions of CD40 and COX-2 in esophageal cancer tissues were significantly correlated (P <0.05), and the correlation coefficient (Φ) was 0.446. MVD in esophageal cancer tissues was significantly higher than that in normal esophageal tissues (25.02 ± 5.52 vs 12.09 ± 4.55, P <0.05). The MVD in CD40 and COX-2 positive group was significantly higher than that in negative group (26.37 ± 6.02 vs 22.58 ± 5.25, P <0.05). The results of in vitro studies showed that the expressions of CD40 and COX-2 in esophageal cancer Eca109 cells were higher than those in normal esophageal epithelial cells (P <0.05). Conclusion: CD40 expression is closely related to the occurrence and development of esophageal squamous cell carcinoma. CD40 may promote the angiogenesis of esophageal carcinoma by affecting the expression of COX-2.