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目的 观察雷公藤多甙防治抗原诱导性葡萄膜视网膜炎(antigen-induceduveore-tinitis,AIU)的临床效果,并探讨免疫学机制。方法 使用牛S抗原和福氏完全佐剂的混合乳剂免疫大鼠14d后,将S抗原接种于玻璃体腔,建立AIU动物模型。实施雷公藤多甙治疗方案,观察其对实验动物的眼部临床表现、病理组织学、血清抗体效价、淋巴细胞增殖反应以及抗原特异性迟发型超敏反应(delayed-typehypersensitivity,DTH)的影响。结果 对照组炎症持续时间平均为12.90d,雷公藤治疗组为7.63d(P<0.01),对照组抗体滴度平均为1∶8～1∶16,雷公藤治疗组为1∶4,受ConA和S抗原刺激的淋巴细胞增殖反应对照组平均为0.68OD和0.84OD,雷公藤治疗组为0.49OD(P<0.01)和0.67OD(P<0.01),对照组DTH平均为1.75mm,雷公藤治疗组为0.66mm(P<0.01)。结论 雷公藤可下调AIU的细胞免疫和体液免疫,具有潜在的应用价值。
Objective To observe the clinical efficacy of tripterygium glycosides in the prevention and treatment of antigen-inducingduveoretinitis (AIU) and to explore the immunological mechanism. Methods After the rats were immunized with mixed emulsion of bovine S antigen and complete Freund’s adjuvant for 14 days, the S antigen was inoculated into vitreous cavity and the animal model of AIU was established. The treatment of tripterygium wilfordii glycosides was carried out to observe the clinical effects, histopathology, serum antibody titer, lymphocyte proliferative response and antigen-specific delayed-type hypersensitivity (DTH) in experimental animals . Results The average duration of inflammation was 12.90 days in the control group and 7.63 days in the tripterygium wilfordii group (P <0.01). The antibody titers in the control group were 1:8 to 1:16 on average and 1: 4 in the triptolide-treated group. (P <0.01) and 0.67 OD (P <0.01). The average DTH in the control group was 1.75 mm, and that of Tripterygium wilfordii The treatment group was 0.66 mm (P <0.01). Conclusion Tripterygium wilfordii can down-regulate cellular immunity and humoral immunity of AIU, which has potential application value.