论文部分内容阅读
应用分子系统发育学的方法,以蟹类18S rDNA和线粒体细胞色素C氧化酶亚单位[(COI)基因序列片段为分子标记,结合形态学特征对10种蟹类的分类地位进行探讨。实验共获得10条18S rDNA序列,长度为1780~1787 bp,其中A、T、G、C平均含量分别为23.72%,24.58%,24.52%和27,17%;通过序列对比,发现18S rDNA序列相对保守,只含有1个从88 bp到130 bp约5()bp的高可变区;物种澡碱基距离比较小,从0.001到0.017。18S rDNA系统发育树为方蟹总科、沙蟹总科和梭子蟹总科起源于同一海洋蟹类祖先提供了分子生物学证据,并且支持将厚蟹属从相手蟹科移至弓蟹科。获得的5条COI基因序列,长度均为709 bp,A、T、G、C平均含量分别为26.88%,37.62%,17.50%和18.00%;COI基因片段变异性高,物种澡碱基距离从0.016到0.138。COⅠ基因系统发育树真实地反映了蟳属各物种之间的进化关系,和传统分类非常吻合,为形态特征相似的蟳类鉴定提供了一种快速准确的方法。
Using molecular phylogenetic analysis, 18S rDNA of crabs and mitochondrial cytochrome C oxidase subunit [(COI) gene fragment were used as molecular markers, and the taxonomic status of 10 species of crabs were analyzed with morphological characteristics. A total of 10 18S rDNA sequences were obtained and the length ranged from 1780 bp to 1787 bp. The average contents of A, T, G and C were 23.72%, 24.58%, 24.52% and 27.17%, respectively. The sequences of 18S rDNA sequences Relatively conservative, containing only one highly variable region from 88 bp to 130 bp, about 5 bp in length. The distance between base and base of the species was relatively small, ranging from 0.001 to 0.017.18S rDNA phylogenetic tree was Crab crab, The Chief and Portulaca salina originated from the same marine crab ancestors providing molecular biological evidence and support for the move of Crab from Crab to Crab. The five COI gene sequences obtained were all 709 bp in length and the average contents of A, T, G and C were 26.88%, 37.62%, 17.50% and 18.00%, respectively. The variability of COI gene fragments was high, 0.016 to 0.138. The phylogenetic tree of COⅠ gene truly reflects the evolutionary relationship among species in Limus, which is in good agreement with the traditional classification and provides a fast and accurate method for the identification of Glycyrrhiza uralensis with similar morphological characteristics.