论文部分内容阅读
利用流动式和间歇式微量热法研究了菲咯啉铜(Ⅱ)-巯基乙醇-O_2体系切割小牛胸腺双链DNA(ds DNA)反应的热力学,确定该DNA断链的总反应为较快的放热反应和焓驱动的反应,但该反应的前期为快速吸热过程,310.15K和pH=7.0时该反应的总摩尔反应焓(△_rH_(m,3))和前期摩尔反应焓(△_rH_(m,1))分别为-35.1和7.29kJ·mol~(-1).同时采用琼脂糖凝胶电泳法观察到了菲咯啉铜(Ⅱ)-巯基乙醇-O_2体系对DNA链的断裂现象.
The thermodynamics of the reaction of copper (Ⅱ) -mercaptoethanol-O_2 system for calf thymus double-stranded DNA (dsDNA) reaction was studied by flow and batch calorimetry. The total reaction of the DNA strand was determined to be faster But the exothermic reaction and enthalpy-driven reaction, but the reaction of the early rapid endothermic process, 310.15K and pH = 7.0 when the total molar reaction enthalpy (△ _rH_ (m, 3)) and pre-molar reaction enthalpy △ _rH_ (m, 1)) were -35.1 and 7.29 kJ · mol -1, respectively.At the same time, the effect of copper (Ⅱ) -mercaptoethanol-O_2 system on the DNA strand was observed by agarose gel electrophoresis Fracture phenomenon.