论文部分内容阅读
目的:探究结缔组织生长因子(Connective tissue growth factor,CTGF)对骨形成蛋白-2(Bone morphogenetic protein-2,BMP-2)诱导PDLCs(periodontal ligament cells,PDLCs)成骨分化能力的影响。方法:采用组织块法分离培养人PDLCs,分3组进行培养:空白组(普通培养基)、对照组(含100 ng/m L BMP-2)和实验组(含100 ng/m L BMP-2+50 ng/m L CTGF)。CCK-8实验检测第1、3、5 d细胞增殖;碱性磷酸酶(alkaline phosphatase,ALP)活性实验检测第5 d ALP活性;qRT-PCR检测第5 d PDLCs中成骨相关基因Runx2、ALP、BSP的mRNA表达量。结果:第3 d实验组细胞数量高于空白组和对照组,第5 d实验组细胞数量显著高于空白组和对照组(P<0.05);实验组ALP活性显著高于对照组和空白组,qRT-PCR结果显示3个成骨相关基因表达实验组均高于空白组和对照组(P<0.05)。结论:CTGF可提高BMP-2诱导PDLCs成骨能力。
Objective: To investigate the effect of connective tissue growth factor (CTGF) on osteogenic differentiation of bone morphogenetic protein-2 (PDLCs) induced by BMP-2. Methods: Human PDLCs were isolated and cultured by tissue block method and cultured in three groups: blank group (normal medium), control group (containing 100 ng / mL BMP-2) and experimental group (containing 100 ng / mL BMP- 2 + 50 ng / mL CTGF). CCK-8 assay detected the proliferation of cells on day 1, 3 and 5; alkaline phosphatase (ALP) activity assayed ALP activity on day 5; qRT-PCR detected osteogenic related gene Runx2 and ALP , BSP mRNA expression levels. Results: The number of cells in the 3rd day was higher than that in the blank group and the control group. The number of cells in the 5th day was significantly higher than that in the blank group and the control group (P <0.05). The ALP activity in the experimental group was significantly higher than that in the control group and the blank group The results of qRT-PCR showed that the expression of three osteogenic genes in experimental group were significantly higher than those in blank group and control group (P <0.05). Conclusion: CTGF can enhance BMP-2-induced osteogenic potential of PDLCs.