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目的 探讨应用电刺激单肌纤维肌电图 (SSFEMG)在不同刺激频率 (5、10、2 0Hz)时检测神经 -肌接头传导功能作为有机磷农药及其混剂中毒引起肌无力效应指标的意义。方法 利用SSFEMG技术观察有机磷类 (乐果、辛硫磷 )、氨基甲酸酯类 (灭多威 )农药及其混剂 (辛硫磷 +灭多威、乐果 +灭多威 )对大鼠神经 -肌接头传导功能的影响 ,并在染毒前和染毒后 1h测定大鼠全血胆碱酯酶 (ChE)活力。结果 各染毒组大鼠肌无力发生的情况为 :乐果 5 / 9,辛硫磷 5 / 10 ,灭多威 0 / 9,辛硫磷+灭多威 8/ 8,乐果 +灭多威 5 / 5 ;肌无力大鼠单肌纤维动作电位平均连续差 (MCD)明显高于对照组 ,辛硫磷和乐果组中的肌无力大鼠MCD明显高于同组肌力正常者 ,差异均有显著性 (P <0 .0 5 )。刺激频率为 10Hz和 2 0Hz的SSFEMG检测结果与大鼠肌无力症状一致性均较好 ;肌无力的出现与ChE的抑制程度不完全一致。结论 有机磷所致肌无力与神经 -肌接头传导功能异常关系密切 ,刺激频率为 10Hz和 2 0Hz的SSFEMG可用作有机磷农药所致肌无力的一个效应指标。
Objective To explore the significance of detecting the conduction of nerve-muscle junction in electrical stimulation of single muscle fiber electromyography (SSFEMG) at different stimulation frequencies (5, 10, and 20 Hz) as an indicator of myasthenia gravis caused by organophosphorus pesticides and their mixtures. Methods SSFEMG technique was used to observe the effects of organophosphorus pesticides (dimethoate, phoxim), carbamates (methomyl) pesticides and their mixtures (phoxim + methomyl, dimethoate and methomyl) Nerve-muscle connector conduction function, and before and 1h after exposure to rat whole blood cholinesterase (ChE) activity. Results The incidence of myasthenia in rats in each exposure group was: dimethoate 5/9, phoxim 5/10, methomyl 0/9, phoxim + methomyl 8/8, dimethoate + methomyl MCD was significantly higher than that of the control group (P <0.05). MCD in phoxim and dimethoate group was significantly higher than that in the same group All were significant (P <0.05). The results of SSFEMG with 10Hz and 20Hz stimulation were consistent with those of rats with myasthenia gravis. The appearance of muscle weakness was not consistent with that of ChE. Conclusions Organophosphorus-induced myasthenia gravis is closely related to the abnormality of neuromuscular junction conduction. SSFEMG with 10 and 20 Hz stimulation frequency can be used as an effect indicator of organophosphate-induced myasthenia gravis.